5 Steps Rare Events Analysis With Flow Cytometry
Poster Jun 29, 2020
Thermo Fisher Scientific
Accurate detection and analysis of rare cells or "events", in a cell population is critical in many fields of research, including cancer and immunology, helping scientists to understand the status and stage of patient's disease. Flow cytometry is the method at the forefront of such analysis and therefore, it's vital to ensure that it is carried out correctly.
Download this poster to discover the five key steps in applying flow cytometry for rare events analysis, helping you to ensure that your results and data are:
Multiplexing cell-based assays is possible using 3D culture models that are larger and more complex than monolayers
Real-time detection methods to measure live or dead cells provide much flexibility for multiplexing
All multiplexed assay combinations should be verified using appropriate controls for each 3D cell culture model.
Basic fibroblast growth factor (bFGF) is widely used in vitro for the maintenance and stimulation of a variety of cells. However, use of native bFGF in cell biology is limited by the fact that bFGF rapidly degrades at physiological temperatures. We have addressed this problem with an engineered form of bFGF, named Heat Stable bFGF (HS bFGF), which is stable at 37 degrees Celsius.READ MORE